Assisted Reproduction Technology In India
Assisted Reproduction Technology of technique is any technological procedure that helps infertile couples to conceive. As per the cause of infertility, our doctors will recommend and perform suitable ART programs.
The actual ART cycle consists of the folllowing procedures : –
- Controlled Ovarian Stimulation
- Oocyte Retrieval
- Laboratory Procedure
- Embryo Transfer
1) Controlled Ovarian Stimulation
The aim of this step is to achieve a higher rate of pregnancy for which the woman’s menstrual cycle is administered. Usually, a woman produces one mature egg during her menstrual cycle. In ART, we aim at initiating the production of several eggs so that several embryos are produced….
The administration of gonadotrophins results in the formation of multiple bags of water in the ovaries, called follicle. Each follicle has within itself, an egg or oocyte. The number and size of the follicles is monitored with the help of serial Vaginal sonography and Estradiol hormone estimation….
3) The Oocyte Retrieval
The oocyte retrieval is done 35 to 37 hours after the maturation injection of HCG. This is a day care outpatient procedure. It may be carried out either under local anesthesia or general anesthesia. We prefer to do it under short general anesthesia, using the latest probofol anesthesia, which has minimal side effects….
4) The Laboratory Procedure
The sperm retrieval : –
Normally, the semen is collected by masturbation 1-2 hours after egg collection…
Semen Production : –
In case the husband has previous history of difficulty in producing semen, we ask him to produce the semen a few days earlier and freeze it, for subsequent use….
Procedures for Semen Retrieval : –
When all of the above methods fail, the patient will be anesthetized for a procedure of electro-ejaculation…
Fertilization : –
The eggs, which are retrieved by aspiration, are then washed with special culture media and kept in incubators. Similarly, the sperms that are obtained are washed with culture media, with the standard swim up method…
5) Embryo Transfer
This is one of the most important steps. The transfer of the selected cleaved embryos is performed 48 hours after the initial egg collection…
Suitable embryos not utilised during the transfer may be cryo-preserved (frozen) for use in another treatment cycle, if desired. These embryos are mixed with special media called cryprotectant. The embryos are loaded into straws, which are then sealed and placed in a special machine called an-Freezer…
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